New cryosolution for stem cell-based therapeutics
Cell-based therapeutics emerged as a critical aspect of regenerative medicine and transfusion. Cryopreservation is the process by which products such as red cells and bone marrow stem cells are stored stably until their use. Unfortunately, current cryopreservation protocols remain suboptimal. Loss of cell viability after freezing dramatically reduce the success of stem cell transplantation and can result in poor outcomes for patients. Current
freezing procedures require the addition of chemicals to prevent the formation of ice that would otherwise rupture cells and decrease the potency of the products. New freezing solutions have been developed that now rely on natural compound rather than chemicals. We hypothesize that the use chemical free solution will reduce cell toxicity and therefore improve the product effectiveness. In this work, we will carefully define the impact of new chemical-free solution on the viability and quality of cells using complementary functional assays and define their impact on the cell physiological status to better understand the relationship between the cryoprotectants and cell potency. In addition, we will test new small ice inhibitors as new additives to prevent ice formation during routine handling of the stem cell grafts. The ensuing improvement in cell products will be of significant benefit to Canadians.
freezing procedures require the addition of chemicals to prevent the formation of ice that would otherwise rupture cells and decrease the potency of the products. New freezing solutions have been developed that now rely on natural compound rather than chemicals. We hypothesize that the use chemical free solution will reduce cell toxicity and therefore improve the product effectiveness. In this work, we will carefully define the impact of new chemical-free solution on the viability and quality of cells using complementary functional assays and define their impact on the cell physiological status to better understand the relationship between the cryoprotectants and cell potency. In addition, we will test new small ice inhibitors as new additives to prevent ice formation during routine handling of the stem cell grafts. The ensuing improvement in cell products will be of significant benefit to Canadians.
Principal Investigator / Supervisor
PINEAULT, Nicolas
Co-Investigator(s) / Trainee
BEN, Robert
HARPER, Marie-Ellen
CHAKRABORTY, Nilay
Institution
Canadian Blood Services
Program
Intramural Research Grant Program
Province
Ontario
Total Amount Awarded
180000.0
Project Start Date
Project End Date